Virus:Article Title: Cav3.1 is a neuronal leucine sensor that mediates satiety and weight loss in response to dietary protein.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Cav3.1 (C-terminal) mouse monoclonal abcam Cat# ab134269 anti-Cav3.1 (C-terminal) rabbit polyclonal abcam Cat# ab203577 anti-Cav3.1 (N-terminal) rabbit polyclonal Alomone Cat# ACC-021; RRID: AB_2039779 anti-cFos rabbit monolonal Synaptic Systems Cat# 226 008; RRID: AB_2891278 anti-cFos guinea Pig monolonal Synaptic Systems Cat# 226 308; RRID: AB_2905595 anti-Phospho-S6 Ribosomal Protein (Ser240/244) rabbit polyclonal Cell Signaling Technology Cat# 2215; RRID: AB_331682 anti-Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) rabbit monoclonal – Alexa Fluor 594 Conjugate Cell Signaling Technology Cat# 9468; RRID: AB_2716873 anti-GFP chicken polyclonal abcam Cat# ab13970; RRID: AB_300798 anti-DsRed mouse monoclonal Takara Bio Cat# 632392; RRID: AB_2801258 anti-POMC rabbit polyclonal Phoenix Pharmaceutical Cat# H-029-30; RRID: AB_2307442 anti-TRPC5 rabbit polyclonal Almone Cat# ACC-020; RRID: AB_2040241 anti-GAPDH mouse monoclonal Santa Cruz Cat# sc-32233; RRID: AB_627679 anti-phospho-p70 S6 Kinase (Thr389) rabbit polyclonal Cell Signaling Technology Cat# 9205; RRID: AB_330944 anti-P70 S6 Kinase rabbit monoclonal Cell Signaling Technology Cat# 2708; RRID: AB_390722 anti-HA mouse monoclonal Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Bacterial and virus strains AAV/DJ-U6-anti-Cacna1g gRNA-CMV-SaCas9 This study; AAV produced by Vector Biolabs N/A AAV/DJ-U6-dual anti-Cacna1g gRNA-hSyn-mCherry This study; AAV produced by Vector Biolabs N/A AAV/DJ-CMV-SaCas9 Vector Biolabs Cat# 7121 AAV/DJ-hSyn-mCherry Vector Biolabs Cat# VB4884 Chemicals, peptides, and recombinant proteins L-Leucine Merck Cat# L8912 aCSF Tocris Cat# 3525 TTA-P2 Almone Cat# T-155 L-Valine Merck Cat# V0513 L-Isoleucine Merck Cat# I7403 Torin 1 Tocris Cat# 4247 SAK3 Tocris Cat# 6239 Recombinant Mouse Leptin R&D Systems Cat# 498-OB Lorcaserin.HCl Generon Cat# M2821 Liraglutide Tocris Cat# 6517 Critical commercial assays RNAscope 2.5 LS Probe Mm-Fos Advanced Cell Diagnostics Cat# 316928 RNAscope 2.5 LS Probe Mm-Trpc5 Advanced Cell Diagnostics Cat# 476248 RNAscope 2.5 LS Probe Mm-Cacna1g-C2 Advanced Cell Diagnostics Cat# 459768-C2 RNAscope 2.5 LS Probe Mm-POMC-C3 Advanced Cell Diagnostics Cat# 314088-C3 Deposited data PhosphoTRAP RNAseq data ArrayExpress E-MTAB-16665 Source Data This paper Data S1 (Continued on next page) ll OPEN ACCESS Article e1 Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hiPSCs FSPS13B A gift from L. Vallier Lab (University of Cambridge) N/A HEK293 ATCC Cat# CRL-1573; RRID: CVCL_0045 mHypoE-N46 Cedarlane Labs Cat# CLU138; RRID: CVCL_D459 Experimental models: Organisms/strains C57BL/6J-Tg(Pomc-EGFP)1Low/J Jackson Laboratory Strain# 009593; RRID: IMSR_ JAX:009593 STOCK Tg(Pomc1-cre)16Lowl/J Jackson Laboratory Strain# 005965; RRID: IMSR_ JAX:005965 B6J.129(B6N)Gt(ROSA)26Sortm1(CAGcas9*,-EGFP)Fezh/J Jackson Laboratory Strain# 026175; RRID: IMSR_ JAX:026175 FVB-Tg(Pomc1-hrGFP)1Lowl/J Jackson Laboratory Strain# 006421; RRID: IMSR_ JAX:006421 129S1/SvImJ-Trpc5tm1.1Clph/J Jackson Laboratory Strain# 030804; RRID: IMSR_ JAX:030804 B6(129S4)-Cacna1gtm1Stl/J Jackson Laboratory Strain# 021932; RRID: IMSR_ JAX:021932 Oligonucleotides Actb; FP: GCTCTGGCTCCTAGCACCAT; RP: GCCACCGATCCACACAGAGT; Probe: ATCAAGATCATTGCTCCTCCTGAGCGC This study; synthesized by Merck N/A Ano6; FP: AGGAGCGCATCCCATTTACC; RP: CGATCACAGAGGCGATGATGA; Probe: CTGTGTGCGAGCGCCGTCTTCTTCTG This study; synthesized by Merck N/A Atg9bFP: GAAGGACTCCGCGGTTC; RP: CCAGCTGGAAGACATCCTC; Probe: CAGGCAAAGCCATTCCGCTGATGATAGC This study; synthesized by Merck N/A Atp6v0a2 Thermo Fisher Scientific Mm00441846_g1 Atxn3; FP: CCATAAGTCGCCAGGAAATCG; RP: AACTACCTTGCATACTGAGCTG; Probe: GGAGGATGAAGAAGCTGATCTCCGCA This study; synthesized by Merck N/A Barhl; FP: AATACCTGAGCGTGCAAGAC; RP: TTCCATTTAGTCCTGCGGTTC; Probe: GGAGCTAGCCGCCTCGCTCA This study; synthesized by Merck N/A Cacna1g Thermo Fisher Scientific Mm00486572_m1 Calca Thermo Fisher Scientific Mm004801463_m1 Calcr Thermo Fisher Scientific Mm00432282_m1 Ccdc164 Thermo Fisher Scientific Mm01210710_m1 Cd38; FP: AACCATACCATGTAACAAGACTCTC; RP: TCATATCAGAAGTACTAGGGTCTCC; Probe:TGGAGGACACCCTGCTGGGC This study; synthesized by Merck N/A Cep110; FP: GCTCCGGCAAATGGATAAGATG; RP: GCATTCTGTCCTTGGTTTGGC; Probe: AAAAGTCCACGAGTGGCGCTTTCAG This study; synthesized by Merck N/A Chrm1; FP: CCTCCCCTATCCACCTTCAGC; RP: GGCATCACCTTGGCATCCAG; Probe: AGGCTGCTGGCCAGATGGACA This study; synthesized by Merck N/A Cln6 Thermo Fisher Scientific Mm01179411_m1 Coch Thermo Fisher Scientific Mm0000483360_m1 Creb3l1; FP: CTCACTGCCCCTCACAAACT; RP: CCTTCTCCTCAGCCTTGGTG; Probe: CATCAGGGCCACTGCTCTTGACAGAAG This study; synthesized by Merck N/A (Continued on next page) ll OPEN ACCESSArticle Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 e2
Bioprocessing:Article Title: Cav3.1 is a neuronal leucine sensor that mediates satiety and weight loss in response to dietary protein.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Cav3.1 (C-terminal) mouse monoclonal abcam Cat# ab134269 anti-Cav3.1 (C-terminal) rabbit polyclonal abcam Cat# ab203577 anti-Cav3.1 (N-terminal) rabbit polyclonal Alomone Cat# ACC-021; RRID: AB_2039779 anti-cFos rabbit monolonal Synaptic Systems Cat# 226 008; RRID: AB_2891278 anti-cFos guinea Pig monolonal Synaptic Systems Cat# 226 308; RRID: AB_2905595 anti-Phospho-S6 Ribosomal Protein (Ser240/244) rabbit polyclonal Cell Signaling Technology Cat# 2215; RRID: AB_331682 anti-Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) rabbit monoclonal – Alexa Fluor 594 Conjugate Cell Signaling Technology Cat# 9468; RRID: AB_2716873 anti-GFP chicken polyclonal abcam Cat# ab13970; RRID: AB_300798 anti-DsRed mouse monoclonal Takara Bio Cat# 632392; RRID: AB_2801258 anti-POMC rabbit polyclonal Phoenix Pharmaceutical Cat# H-029-30; RRID: AB_2307442 anti-TRPC5 rabbit polyclonal Almone Cat# ACC-020; RRID: AB_2040241 anti-GAPDH mouse monoclonal Santa Cruz Cat# sc-32233; RRID: AB_627679 anti-phospho-p70 S6 Kinase (Thr389) rabbit polyclonal Cell Signaling Technology Cat# 9205; RRID: AB_330944 anti-P70 S6 Kinase rabbit monoclonal Cell Signaling Technology Cat# 2708; RRID: AB_390722 anti-HA mouse monoclonal Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Bacterial and virus strains AAV/DJ-U6-anti-Cacna1g gRNA-CMV-SaCas9 This study; AAV produced by Vector Biolabs N/A AAV/DJ-U6-dual anti-Cacna1g gRNA-hSyn-mCherry This study; AAV produced by Vector Biolabs N/A AAV/DJ-CMV-SaCas9 Vector Biolabs Cat# 7121 AAV/DJ-hSyn-mCherry Vector Biolabs Cat# VB4884 Chemicals, peptides, and recombinant proteins L-Leucine Merck Cat# L8912 aCSF Tocris Cat# 3525 TTA-P2 Almone Cat# T-155 L-Valine Merck Cat# V0513 L-Isoleucine Merck Cat# I7403 Torin 1 Tocris Cat# 4247 SAK3 Tocris Cat# 6239 Recombinant Mouse Leptin R&D Systems Cat# 498-OB Lorcaserin.HCl Generon Cat# M2821 Liraglutide Tocris Cat# 6517 Critical commercial assays RNAscope 2.5 LS Probe Mm-Fos Advanced Cell Diagnostics Cat# 316928 RNAscope 2.5 LS Probe Mm-Trpc5 Advanced Cell Diagnostics Cat# 476248 RNAscope 2.5 LS Probe Mm-Cacna1g-C2 Advanced Cell Diagnostics Cat# 459768-C2 RNAscope 2.5 LS Probe Mm-POMC-C3 Advanced Cell Diagnostics Cat# 314088-C3 Deposited data PhosphoTRAP RNAseq data ArrayExpress E-MTAB-16665 Source Data This paper Data S1 (Continued on next page) ll OPEN ACCESS Article e1 Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hiPSCs FSPS13B A gift from L. Vallier Lab (University of Cambridge) N/A HEK293 ATCC Cat# CRL-1573; RRID: CVCL_0045 mHypoE-N46 Cedarlane Labs Cat# CLU138; RRID: CVCL_D459 Experimental models: Organisms/strains C57BL/6J-Tg(Pomc-EGFP)1Low/J Jackson Laboratory Strain# 009593; RRID: IMSR_ JAX:009593 STOCK Tg(Pomc1-cre)16Lowl/J Jackson Laboratory Strain# 005965; RRID: IMSR_ JAX:005965 B6J.129(B6N)Gt(ROSA)26Sortm1(CAGcas9*,-EGFP)Fezh/J Jackson Laboratory Strain# 026175; RRID: IMSR_ JAX:026175 FVB-Tg(Pomc1-hrGFP)1Lowl/J Jackson Laboratory Strain# 006421; RRID: IMSR_ JAX:006421 129S1/SvImJ-Trpc5tm1.1Clph/J Jackson Laboratory Strain# 030804; RRID: IMSR_ JAX:030804 B6(129S4)-Cacna1gtm1Stl/J Jackson Laboratory Strain# 021932; RRID: IMSR_ JAX:021932 Oligonucleotides Actb; FP: GCTCTGGCTCCTAGCACCAT; RP: GCCACCGATCCACACAGAGT; Probe: ATCAAGATCATTGCTCCTCCTGAGCGC This study; synthesized by Merck N/A Ano6; FP: AGGAGCGCATCCCATTTACC; RP: CGATCACAGAGGCGATGATGA; Probe: CTGTGTGCGAGCGCCGTCTTCTTCTG This study; synthesized by Merck N/A Atg9bFP: GAAGGACTCCGCGGTTC; RP: CCAGCTGGAAGACATCCTC; Probe: CAGGCAAAGCCATTCCGCTGATGATAGC This study; synthesized by Merck N/A Atp6v0a2 Thermo Fisher Scientific Mm00441846_g1 Atxn3; FP: CCATAAGTCGCCAGGAAATCG; RP: AACTACCTTGCATACTGAGCTG; Probe: GGAGGATGAAGAAGCTGATCTCCGCA This study; synthesized by Merck N/A Barhl; FP: AATACCTGAGCGTGCAAGAC; RP: TTCCATTTAGTCCTGCGGTTC; Probe: GGAGCTAGCCGCCTCGCTCA This study; synthesized by Merck N/A Cacna1g Thermo Fisher Scientific Mm00486572_m1 Calca Thermo Fisher Scientific Mm004801463_m1 Calcr Thermo Fisher Scientific Mm00432282_m1 Ccdc164 Thermo Fisher Scientific Mm01210710_m1 Cd38; FP: AACCATACCATGTAACAAGACTCTC; RP: TCATATCAGAAGTACTAGGGTCTCC; Probe:TGGAGGACACCCTGCTGGGC This study; synthesized by Merck N/A Cep110; FP: GCTCCGGCAAATGGATAAGATG; RP: GCATTCTGTCCTTGGTTTGGC; Probe: AAAAGTCCACGAGTGGCGCTTTCAG This study; synthesized by Merck N/A Chrm1; FP: CCTCCCCTATCCACCTTCAGC; RP: GGCATCACCTTGGCATCCAG; Probe: AGGCTGCTGGCCAGATGGACA This study; synthesized by Merck N/A Cln6 Thermo Fisher Scientific Mm01179411_m1 Coch Thermo Fisher Scientific Mm0000483360_m1 Creb3l1; FP: CTCACTGCCCCTCACAAACT; RP: CCTTCTCCTCAGCCTTGGTG; Probe: CATCAGGGCCACTGCTCTTGACAGAAG This study; synthesized by Merck N/A (Continued on next page) ll OPEN ACCESSArticle Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 e2
Produced:Article Title: Cav3.1 is a neuronal leucine sensor that mediates satiety and weight loss in response to dietary protein.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Cav3.1 (C-terminal) mouse monoclonal abcam Cat# ab134269 anti-Cav3.1 (C-terminal) rabbit polyclonal abcam Cat# ab203577 anti-Cav3.1 (N-terminal) rabbit polyclonal Alomone Cat# ACC-021; RRID: AB_2039779 anti-cFos rabbit monolonal Synaptic Systems Cat# 226 008; RRID: AB_2891278 anti-cFos guinea Pig monolonal Synaptic Systems Cat# 226 308; RRID: AB_2905595 anti-Phospho-S6 Ribosomal Protein (Ser240/244) rabbit polyclonal Cell Signaling Technology Cat# 2215; RRID: AB_331682 anti-Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) rabbit monoclonal – Alexa Fluor 594 Conjugate Cell Signaling Technology Cat# 9468; RRID: AB_2716873 anti-GFP chicken polyclonal abcam Cat# ab13970; RRID: AB_300798 anti-DsRed mouse monoclonal Takara Bio Cat# 632392; RRID: AB_2801258 anti-POMC rabbit polyclonal Phoenix Pharmaceutical Cat# H-029-30; RRID: AB_2307442 anti-TRPC5 rabbit polyclonal Almone Cat# ACC-020; RRID: AB_2040241 anti-GAPDH mouse monoclonal Santa Cruz Cat# sc-32233; RRID: AB_627679 anti-phospho-p70 S6 Kinase (Thr389) rabbit polyclonal Cell Signaling Technology Cat# 9205; RRID: AB_330944 anti-P70 S6 Kinase rabbit monoclonal Cell Signaling Technology Cat# 2708; RRID: AB_390722 anti-HA mouse monoclonal Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Bacterial and virus strains AAV/DJ-U6-anti-Cacna1g gRNA-CMV-SaCas9 This study; AAV produced by Vector Biolabs N/A AAV/DJ-U6-dual anti-Cacna1g gRNA-hSyn-mCherry This study; AAV produced by Vector Biolabs N/A AAV/DJ-CMV-SaCas9 Vector Biolabs Cat# 7121 AAV/DJ-hSyn-mCherry Vector Biolabs Cat# VB4884 Chemicals, peptides, and recombinant proteins L-Leucine Merck Cat# L8912 aCSF Tocris Cat# 3525 TTA-P2 Almone Cat# T-155 L-Valine Merck Cat# V0513 L-Isoleucine Merck Cat# I7403 Torin 1 Tocris Cat# 4247 SAK3 Tocris Cat# 6239 Recombinant Mouse Leptin R&D Systems Cat# 498-OB Lorcaserin.HCl Generon Cat# M2821 Liraglutide Tocris Cat# 6517 Critical commercial assays RNAscope 2.5 LS Probe Mm-Fos Advanced Cell Diagnostics Cat# 316928 RNAscope 2.5 LS Probe Mm-Trpc5 Advanced Cell Diagnostics Cat# 476248 RNAscope 2.5 LS Probe Mm-Cacna1g-C2 Advanced Cell Diagnostics Cat# 459768-C2 RNAscope 2.5 LS Probe Mm-POMC-C3 Advanced Cell Diagnostics Cat# 314088-C3 Deposited data PhosphoTRAP RNAseq data ArrayExpress E-MTAB-16665 Source Data This paper Data S1 (Continued on next page) ll OPEN ACCESS Article e1 Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hiPSCs FSPS13B A gift from L. Vallier Lab (University of Cambridge) N/A HEK293 ATCC Cat# CRL-1573; RRID: CVCL_0045 mHypoE-N46 Cedarlane Labs Cat# CLU138; RRID: CVCL_D459 Experimental models: Organisms/strains C57BL/6J-Tg(Pomc-EGFP)1Low/J Jackson Laboratory Strain# 009593; RRID: IMSR_ JAX:009593 STOCK Tg(Pomc1-cre)16Lowl/J Jackson Laboratory Strain# 005965; RRID: IMSR_ JAX:005965 B6J.129(B6N)Gt(ROSA)26Sortm1(CAGcas9*,-EGFP)Fezh/J Jackson Laboratory Strain# 026175; RRID: IMSR_ JAX:026175 FVB-Tg(Pomc1-hrGFP)1Lowl/J Jackson Laboratory Strain# 006421; RRID: IMSR_ JAX:006421 129S1/SvImJ-Trpc5tm1.1Clph/J Jackson Laboratory Strain# 030804; RRID: IMSR_ JAX:030804 B6(129S4)-Cacna1gtm1Stl/J Jackson Laboratory Strain# 021932; RRID: IMSR_ JAX:021932 Oligonucleotides Actb; FP: GCTCTGGCTCCTAGCACCAT; RP: GCCACCGATCCACACAGAGT; Probe: ATCAAGATCATTGCTCCTCCTGAGCGC This study; synthesized by Merck N/A Ano6; FP: AGGAGCGCATCCCATTTACC; RP: CGATCACAGAGGCGATGATGA; Probe: CTGTGTGCGAGCGCCGTCTTCTTCTG This study; synthesized by Merck N/A Atg9bFP: GAAGGACTCCGCGGTTC; RP: CCAGCTGGAAGACATCCTC; Probe: CAGGCAAAGCCATTCCGCTGATGATAGC This study; synthesized by Merck N/A Atp6v0a2 Thermo Fisher Scientific Mm00441846_g1 Atxn3; FP: CCATAAGTCGCCAGGAAATCG; RP: AACTACCTTGCATACTGAGCTG; Probe: GGAGGATGAAGAAGCTGATCTCCGCA This study; synthesized by Merck N/A Barhl; FP: AATACCTGAGCGTGCAAGAC; RP: TTCCATTTAGTCCTGCGGTTC; Probe: GGAGCTAGCCGCCTCGCTCA This study; synthesized by Merck N/A Cacna1g Thermo Fisher Scientific Mm00486572_m1 Calca Thermo Fisher Scientific Mm004801463_m1 Calcr Thermo Fisher Scientific Mm00432282_m1 Ccdc164 Thermo Fisher Scientific Mm01210710_m1 Cd38; FP: AACCATACCATGTAACAAGACTCTC; RP: TCATATCAGAAGTACTAGGGTCTCC; Probe:TGGAGGACACCCTGCTGGGC This study; synthesized by Merck N/A Cep110; FP: GCTCCGGCAAATGGATAAGATG; RP: GCATTCTGTCCTTGGTTTGGC; Probe: AAAAGTCCACGAGTGGCGCTTTCAG This study; synthesized by Merck N/A Chrm1; FP: CCTCCCCTATCCACCTTCAGC; RP: GGCATCACCTTGGCATCCAG; Probe: AGGCTGCTGGCCAGATGGACA This study; synthesized by Merck N/A Cln6 Thermo Fisher Scientific Mm01179411_m1 Coch Thermo Fisher Scientific Mm0000483360_m1 Creb3l1; FP: CTCACTGCCCCTCACAAACT; RP: CCTTCTCCTCAGCCTTGGTG; Probe: CATCAGGGCCACTGCTCTTGACAGAAG This study; synthesized by Merck N/A (Continued on next page) ll OPEN ACCESSArticle Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 e2
Recombinant:Article Title: Cav3.1 is a neuronal leucine sensor that mediates satiety and weight loss in response to dietary protein.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Cav3.1 (C-terminal) mouse monoclonal abcam Cat# ab134269 anti-Cav3.1 (C-terminal) rabbit polyclonal abcam Cat# ab203577 anti-Cav3.1 (N-terminal) rabbit polyclonal Alomone Cat# ACC-021; RRID: AB_2039779 anti-cFos rabbit monolonal Synaptic Systems Cat# 226 008; RRID: AB_2891278 anti-cFos guinea Pig monolonal Synaptic Systems Cat# 226 308; RRID: AB_2905595 anti-Phospho-S6 Ribosomal Protein (Ser240/244) rabbit polyclonal Cell Signaling Technology Cat# 2215; RRID: AB_331682 anti-Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) rabbit monoclonal – Alexa Fluor 594 Conjugate Cell Signaling Technology Cat# 9468; RRID: AB_2716873 anti-GFP chicken polyclonal abcam Cat# ab13970; RRID: AB_300798 anti-DsRed mouse monoclonal Takara Bio Cat# 632392; RRID: AB_2801258 anti-POMC rabbit polyclonal Phoenix Pharmaceutical Cat# H-029-30; RRID: AB_2307442 anti-TRPC5 rabbit polyclonal Almone Cat# ACC-020; RRID: AB_2040241 anti-GAPDH mouse monoclonal Santa Cruz Cat# sc-32233; RRID: AB_627679 anti-phospho-p70 S6 Kinase (Thr389) rabbit polyclonal Cell Signaling Technology Cat# 9205; RRID: AB_330944 anti-P70 S6 Kinase rabbit monoclonal Cell Signaling Technology Cat# 2708; RRID: AB_390722 anti-HA mouse monoclonal Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Bacterial and virus strains AAV/DJ-U6-anti-Cacna1g gRNA-CMV-SaCas9 This study; AAV produced by Vector Biolabs N/A AAV/DJ-U6-dual anti-Cacna1g gRNA-hSyn-mCherry This study; AAV produced by Vector Biolabs N/A AAV/DJ-CMV-SaCas9 Vector Biolabs Cat# 7121 AAV/DJ-hSyn-mCherry Vector Biolabs Cat# VB4884 Chemicals, peptides, and recombinant proteins L-Leucine Merck Cat# L8912 aCSF Tocris Cat# 3525 TTA-P2 Almone Cat# T-155 L-Valine Merck Cat# V0513 L-Isoleucine Merck Cat# I7403 Torin 1 Tocris Cat# 4247 SAK3 Tocris Cat# 6239 Recombinant Mouse Leptin R&D Systems Cat# 498-OB Lorcaserin.HCl Generon Cat# M2821 Liraglutide Tocris Cat# 6517 Critical commercial assays RNAscope 2.5 LS Probe Mm-Fos Advanced Cell Diagnostics Cat# 316928 RNAscope 2.5 LS Probe Mm-Trpc5 Advanced Cell Diagnostics Cat# 476248 RNAscope 2.5 LS Probe Mm-Cacna1g-C2 Advanced Cell Diagnostics Cat# 459768-C2 RNAscope 2.5 LS Probe Mm-POMC-C3 Advanced Cell Diagnostics Cat# 314088-C3 Deposited data PhosphoTRAP RNAseq data ArrayExpress E-MTAB-16665 Source Data This paper Data S1 (Continued on next page) ll OPEN ACCESS Article e1 Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hiPSCs FSPS13B A gift from L. Vallier Lab (University of Cambridge) N/A HEK293 ATCC Cat# CRL-1573; RRID: CVCL_0045 mHypoE-N46 Cedarlane Labs Cat# CLU138; RRID: CVCL_D459 Experimental models: Organisms/strains C57BL/6J-Tg(Pomc-EGFP)1Low/J Jackson Laboratory Strain# 009593; RRID: IMSR_ JAX:009593 STOCK Tg(Pomc1-cre)16Lowl/J Jackson Laboratory Strain# 005965; RRID: IMSR_ JAX:005965 B6J.129(B6N)Gt(ROSA)26Sortm1(CAGcas9*,-EGFP)Fezh/J Jackson Laboratory Strain# 026175; RRID: IMSR_ JAX:026175 FVB-Tg(Pomc1-hrGFP)1Lowl/J Jackson Laboratory Strain# 006421; RRID: IMSR_ JAX:006421 129S1/SvImJ-Trpc5tm1.1Clph/J Jackson Laboratory Strain# 030804; RRID: IMSR_ JAX:030804 B6(129S4)-Cacna1gtm1Stl/J Jackson Laboratory Strain# 021932; RRID: IMSR_ JAX:021932 Oligonucleotides Actb; FP: GCTCTGGCTCCTAGCACCAT; RP: GCCACCGATCCACACAGAGT; Probe: ATCAAGATCATTGCTCCTCCTGAGCGC This study; synthesized by Merck N/A Ano6; FP: AGGAGCGCATCCCATTTACC; RP: CGATCACAGAGGCGATGATGA; Probe: CTGTGTGCGAGCGCCGTCTTCTTCTG This study; synthesized by Merck N/A Atg9bFP: GAAGGACTCCGCGGTTC; RP: CCAGCTGGAAGACATCCTC; Probe: CAGGCAAAGCCATTCCGCTGATGATAGC This study; synthesized by Merck N/A Atp6v0a2 Thermo Fisher Scientific Mm00441846_g1 Atxn3; FP: CCATAAGTCGCCAGGAAATCG; RP: AACTACCTTGCATACTGAGCTG; Probe: GGAGGATGAAGAAGCTGATCTCCGCA This study; synthesized by Merck N/A Barhl; FP: AATACCTGAGCGTGCAAGAC; RP: TTCCATTTAGTCCTGCGGTTC; Probe: GGAGCTAGCCGCCTCGCTCA This study; synthesized by Merck N/A Cacna1g Thermo Fisher Scientific Mm00486572_m1 Calca Thermo Fisher Scientific Mm004801463_m1 Calcr Thermo Fisher Scientific Mm00432282_m1 Ccdc164 Thermo Fisher Scientific Mm01210710_m1 Cd38; FP: AACCATACCATGTAACAAGACTCTC; RP: TCATATCAGAAGTACTAGGGTCTCC; Probe:TGGAGGACACCCTGCTGGGC This study; synthesized by Merck N/A Cep110; FP: GCTCCGGCAAATGGATAAGATG; RP: GCATTCTGTCCTTGGTTTGGC; Probe: AAAAGTCCACGAGTGGCGCTTTCAG This study; synthesized by Merck N/A Chrm1; FP: CCTCCCCTATCCACCTTCAGC; RP: GGCATCACCTTGGCATCCAG; Probe: AGGCTGCTGGCCAGATGGACA This study; synthesized by Merck N/A Cln6 Thermo Fisher Scientific Mm01179411_m1 Coch Thermo Fisher Scientific Mm0000483360_m1 Creb3l1; FP: CTCACTGCCCCTCACAAACT; RP: CCTTCTCCTCAGCCTTGGTG; Probe: CATCAGGGCCACTGCTCTTGACAGAAG This study; synthesized by Merck N/A (Continued on next page) ll OPEN ACCESSArticle Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 e2
RNAscope:Article Title: Cav3.1 is a neuronal leucine sensor that mediates satiety and weight loss in response to dietary protein.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Cav3.1 (C-terminal) mouse monoclonal abcam Cat# ab134269 anti-Cav3.1 (C-terminal) rabbit polyclonal abcam Cat# ab203577 anti-Cav3.1 (N-terminal) rabbit polyclonal Alomone Cat# ACC-021; RRID: AB_2039779 anti-cFos rabbit monolonal Synaptic Systems Cat# 226 008; RRID: AB_2891278 anti-cFos guinea Pig monolonal Synaptic Systems Cat# 226 308; RRID: AB_2905595 anti-Phospho-S6 Ribosomal Protein (Ser240/244) rabbit polyclonal Cell Signaling Technology Cat# 2215; RRID: AB_331682 anti-Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) rabbit monoclonal – Alexa Fluor 594 Conjugate Cell Signaling Technology Cat# 9468; RRID: AB_2716873 anti-GFP chicken polyclonal abcam Cat# ab13970; RRID: AB_300798 anti-DsRed mouse monoclonal Takara Bio Cat# 632392; RRID: AB_2801258 anti-POMC rabbit polyclonal Phoenix Pharmaceutical Cat# H-029-30; RRID: AB_2307442 anti-TRPC5 rabbit polyclonal Almone Cat# ACC-020; RRID: AB_2040241 anti-GAPDH mouse monoclonal Santa Cruz Cat# sc-32233; RRID: AB_627679 anti-phospho-p70 S6 Kinase (Thr389) rabbit polyclonal Cell Signaling Technology Cat# 9205; RRID: AB_330944 anti-P70 S6 Kinase rabbit monoclonal Cell Signaling Technology Cat# 2708; RRID: AB_390722 anti-HA mouse monoclonal Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Bacterial and virus strains AAV/DJ-U6-anti-Cacna1g gRNA-CMV-SaCas9 This study; AAV produced by Vector Biolabs N/A AAV/DJ-U6-dual anti-Cacna1g gRNA-hSyn-mCherry This study; AAV produced by Vector Biolabs N/A AAV/DJ-CMV-SaCas9 Vector Biolabs Cat# 7121 AAV/DJ-hSyn-mCherry Vector Biolabs Cat# VB4884 Chemicals, peptides, and recombinant proteins L-Leucine Merck Cat# L8912 aCSF Tocris Cat# 3525 TTA-P2 Almone Cat# T-155 L-Valine Merck Cat# V0513 L-Isoleucine Merck Cat# I7403 Torin 1 Tocris Cat# 4247 SAK3 Tocris Cat# 6239 Recombinant Mouse Leptin R&D Systems Cat# 498-OB Lorcaserin.HCl Generon Cat# M2821 Liraglutide Tocris Cat# 6517 Critical commercial assays RNAscope 2.5 LS Probe Mm-Fos Advanced Cell Diagnostics Cat# 316928 RNAscope 2.5 LS Probe Mm-Trpc5 Advanced Cell Diagnostics Cat# 476248 RNAscope 2.5 LS Probe Mm-Cacna1g-C2 Advanced Cell Diagnostics Cat# 459768-C2 RNAscope 2.5 LS Probe Mm-POMC-C3 Advanced Cell Diagnostics Cat# 314088-C3 Deposited data PhosphoTRAP RNAseq data ArrayExpress E-MTAB-16665 Source Data This paper Data S1 (Continued on next page) ll OPEN ACCESS Article e1 Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hiPSCs FSPS13B A gift from L. Vallier Lab (University of Cambridge) N/A HEK293 ATCC Cat# CRL-1573; RRID: CVCL_0045 mHypoE-N46 Cedarlane Labs Cat# CLU138; RRID: CVCL_D459 Experimental models: Organisms/strains C57BL/6J-Tg(Pomc-EGFP)1Low/J Jackson Laboratory Strain# 009593; RRID: IMSR_ JAX:009593 STOCK Tg(Pomc1-cre)16Lowl/J Jackson Laboratory Strain# 005965; RRID: IMSR_ JAX:005965 B6J.129(B6N)Gt(ROSA)26Sortm1(CAGcas9*,-EGFP)Fezh/J Jackson Laboratory Strain# 026175; RRID: IMSR_ JAX:026175 FVB-Tg(Pomc1-hrGFP)1Lowl/J Jackson Laboratory Strain# 006421; RRID: IMSR_ JAX:006421 129S1/SvImJ-Trpc5tm1.1Clph/J Jackson Laboratory Strain# 030804; RRID: IMSR_ JAX:030804 B6(129S4)-Cacna1gtm1Stl/J Jackson Laboratory Strain# 021932; RRID: IMSR_ JAX:021932 Oligonucleotides Actb; FP: GCTCTGGCTCCTAGCACCAT; RP: GCCACCGATCCACACAGAGT; Probe: ATCAAGATCATTGCTCCTCCTGAGCGC This study; synthesized by Merck N/A Ano6; FP: AGGAGCGCATCCCATTTACC; RP: CGATCACAGAGGCGATGATGA; Probe: CTGTGTGCGAGCGCCGTCTTCTTCTG This study; synthesized by Merck N/A Atg9bFP: GAAGGACTCCGCGGTTC; RP: CCAGCTGGAAGACATCCTC; Probe: CAGGCAAAGCCATTCCGCTGATGATAGC This study; synthesized by Merck N/A Atp6v0a2 Thermo Fisher Scientific Mm00441846_g1 Atxn3; FP: CCATAAGTCGCCAGGAAATCG; RP: AACTACCTTGCATACTGAGCTG; Probe: GGAGGATGAAGAAGCTGATCTCCGCA This study; synthesized by Merck N/A Barhl; FP: AATACCTGAGCGTGCAAGAC; RP: TTCCATTTAGTCCTGCGGTTC; Probe: GGAGCTAGCCGCCTCGCTCA This study; synthesized by Merck N/A Cacna1g Thermo Fisher Scientific Mm00486572_m1 Calca Thermo Fisher Scientific Mm004801463_m1 Calcr Thermo Fisher Scientific Mm00432282_m1 Ccdc164 Thermo Fisher Scientific Mm01210710_m1 Cd38; FP: AACCATACCATGTAACAAGACTCTC; RP: TCATATCAGAAGTACTAGGGTCTCC; Probe:TGGAGGACACCCTGCTGGGC This study; synthesized by Merck N/A Cep110; FP: GCTCCGGCAAATGGATAAGATG; RP: GCATTCTGTCCTTGGTTTGGC; Probe: AAAAGTCCACGAGTGGCGCTTTCAG This study; synthesized by Merck N/A Chrm1; FP: CCTCCCCTATCCACCTTCAGC; RP: GGCATCACCTTGGCATCCAG; Probe: AGGCTGCTGGCCAGATGGACA This study; synthesized by Merck N/A Cln6 Thermo Fisher Scientific Mm01179411_m1 Coch Thermo Fisher Scientific Mm0000483360_m1 Creb3l1; FP: CTCACTGCCCCTCACAAACT; RP: CCTTCTCCTCAGCCTTGGTG; Probe: CATCAGGGCCACTGCTCTTGACAGAAG This study; synthesized by Merck N/A (Continued on next page) ll OPEN ACCESSArticle Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 e2
RNA sequencing:Article Title: Cav3.1 is a neuronal leucine sensor that mediates satiety and weight loss in response to dietary protein.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Cav3.1 (C-terminal) mouse monoclonal abcam Cat# ab134269 anti-Cav3.1 (C-terminal) rabbit polyclonal abcam Cat# ab203577 anti-Cav3.1 (N-terminal) rabbit polyclonal Alomone Cat# ACC-021; RRID: AB_2039779 anti-cFos rabbit monolonal Synaptic Systems Cat# 226 008; RRID: AB_2891278 anti-cFos guinea Pig monolonal Synaptic Systems Cat# 226 308; RRID: AB_2905595 anti-Phospho-S6 Ribosomal Protein (Ser240/244) rabbit polyclonal Cell Signaling Technology Cat# 2215; RRID: AB_331682 anti-Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) rabbit monoclonal – Alexa Fluor 594 Conjugate Cell Signaling Technology Cat# 9468; RRID: AB_2716873 anti-GFP chicken polyclonal abcam Cat# ab13970; RRID: AB_300798 anti-DsRed mouse monoclonal Takara Bio Cat# 632392; RRID: AB_2801258 anti-POMC rabbit polyclonal Phoenix Pharmaceutical Cat# H-029-30; RRID: AB_2307442 anti-TRPC5 rabbit polyclonal Almone Cat# ACC-020; RRID: AB_2040241 anti-GAPDH mouse monoclonal Santa Cruz Cat# sc-32233; RRID: AB_627679 anti-phospho-p70 S6 Kinase (Thr389) rabbit polyclonal Cell Signaling Technology Cat# 9205; RRID: AB_330944 anti-P70 S6 Kinase rabbit monoclonal Cell Signaling Technology Cat# 2708; RRID: AB_390722 anti-HA mouse monoclonal Cell Signaling Technology Cat# 2367; RRID: AB_10691311 Bacterial and virus strains AAV/DJ-U6-anti-Cacna1g gRNA-CMV-SaCas9 This study; AAV produced by Vector Biolabs N/A AAV/DJ-U6-dual anti-Cacna1g gRNA-hSyn-mCherry This study; AAV produced by Vector Biolabs N/A AAV/DJ-CMV-SaCas9 Vector Biolabs Cat# 7121 AAV/DJ-hSyn-mCherry Vector Biolabs Cat# VB4884 Chemicals, peptides, and recombinant proteins L-Leucine Merck Cat# L8912 aCSF Tocris Cat# 3525 TTA-P2 Almone Cat# T-155 L-Valine Merck Cat# V0513 L-Isoleucine Merck Cat# I7403 Torin 1 Tocris Cat# 4247 SAK3 Tocris Cat# 6239 Recombinant Mouse Leptin R&D Systems Cat# 498-OB Lorcaserin.HCl Generon Cat# M2821 Liraglutide Tocris Cat# 6517 Critical commercial assays RNAscope 2.5 LS Probe Mm-Fos Advanced Cell Diagnostics Cat# 316928 RNAscope 2.5 LS Probe Mm-Trpc5 Advanced Cell Diagnostics Cat# 476248 RNAscope 2.5 LS Probe Mm-Cacna1g-C2 Advanced Cell Diagnostics Cat# 459768-C2 RNAscope 2.5 LS Probe Mm-POMC-C3 Advanced Cell Diagnostics Cat# 314088-C3 Deposited data PhosphoTRAP RNAseq data ArrayExpress E-MTAB-16665 Source Data This paper Data S1 (Continued on next page) ll OPEN ACCESS Article e1 Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines hiPSCs FSPS13B A gift from L. Vallier Lab (University of Cambridge) N/A HEK293 ATCC Cat# CRL-1573; RRID: CVCL_0045 mHypoE-N46 Cedarlane Labs Cat# CLU138; RRID: CVCL_D459 Experimental models: Organisms/strains C57BL/6J-Tg(Pomc-EGFP)1Low/J Jackson Laboratory Strain# 009593; RRID: IMSR_ JAX:009593 STOCK Tg(Pomc1-cre)16Lowl/J Jackson Laboratory Strain# 005965; RRID: IMSR_ JAX:005965 B6J.129(B6N)Gt(ROSA)26Sortm1(CAGcas9*,-EGFP)Fezh/J Jackson Laboratory Strain# 026175; RRID: IMSR_ JAX:026175 FVB-Tg(Pomc1-hrGFP)1Lowl/J Jackson Laboratory Strain# 006421; RRID: IMSR_ JAX:006421 129S1/SvImJ-Trpc5tm1.1Clph/J Jackson Laboratory Strain# 030804; RRID: IMSR_ JAX:030804 B6(129S4)-Cacna1gtm1Stl/J Jackson Laboratory Strain# 021932; RRID: IMSR_ JAX:021932 Oligonucleotides Actb; FP: GCTCTGGCTCCTAGCACCAT; RP: GCCACCGATCCACACAGAGT; Probe: ATCAAGATCATTGCTCCTCCTGAGCGC This study; synthesized by Merck N/A Ano6; FP: AGGAGCGCATCCCATTTACC; RP: CGATCACAGAGGCGATGATGA; Probe: CTGTGTGCGAGCGCCGTCTTCTTCTG This study; synthesized by Merck N/A Atg9bFP: GAAGGACTCCGCGGTTC; RP: CCAGCTGGAAGACATCCTC; Probe: CAGGCAAAGCCATTCCGCTGATGATAGC This study; synthesized by Merck N/A Atp6v0a2 Thermo Fisher Scientific Mm00441846_g1 Atxn3; FP: CCATAAGTCGCCAGGAAATCG; RP: AACTACCTTGCATACTGAGCTG; Probe: GGAGGATGAAGAAGCTGATCTCCGCA This study; synthesized by Merck N/A Barhl; FP: AATACCTGAGCGTGCAAGAC; RP: TTCCATTTAGTCCTGCGGTTC; Probe: GGAGCTAGCCGCCTCGCTCA This study; synthesized by Merck N/A Cacna1g Thermo Fisher Scientific Mm00486572_m1 Calca Thermo Fisher Scientific Mm004801463_m1 Calcr Thermo Fisher Scientific Mm00432282_m1 Ccdc164 Thermo Fisher Scientific Mm01210710_m1 Cd38; FP: AACCATACCATGTAACAAGACTCTC; RP: TCATATCAGAAGTACTAGGGTCTCC; Probe:TGGAGGACACCCTGCTGGGC This study; synthesized by Merck N/A Cep110; FP: GCTCCGGCAAATGGATAAGATG; RP: GCATTCTGTCCTTGGTTTGGC; Probe: AAAAGTCCACGAGTGGCGCTTTCAG This study; synthesized by Merck N/A Chrm1; FP: CCTCCCCTATCCACCTTCAGC; RP: GGCATCACCTTGGCATCCAG; Probe: AGGCTGCTGGCCAGATGGACA This study; synthesized by Merck N/A Cln6 Thermo Fisher Scientific Mm01179411_m1 Coch Thermo Fisher Scientific Mm0000483360_m1 Creb3l1; FP: CTCACTGCCCCTCACAAACT; RP: CCTTCTCCTCAGCCTTGGTG; Probe: CATCAGGGCCACTGCTCTTGACAGAAG This study; synthesized by Merck N/A (Continued on next page) ll OPEN ACCESSArticle Cell Metabolism 38, 1–15.e1–e13, May 5, 2026 e2
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